EFEK ROSELLA MERAH KERING TERHADAP KADAR MDA SERUM TIKUS STRAIN WISTAR YANG DIPAPAR ASAP ROKOK

Novera Herdiani, Wiwik Afridah

Abstract


Abstract

Cigarette smoke contains free radicals. Currently the number of smokers in Indonesia are still high. This study aims to analyze the effects of red rosella of dried on the prevention of the increase of Malondialdehyde (MDA) serum of strain wistar rats exposed to cigarette smoke. The total sample of 24 male rats and divided into four groups : negative control, positive control, treatment of red rosella on dried dose 540 mg/bw, and red rosella on dried dose 810 mg/bw. Negative control only given with standard feed. Positive control given by standard feed and exposed to 2 cigarettes a day. Treatment group were feed by standard and red rosella of dried in the morning and exposed to 2 cigarettes after that. This study was conducted for 21 days. At the end of the study, blood serum was analyzed to determine MDA. The results of the study of MDA serum test is analyzed with One Way ANOVA and followed by Tukey LSD test at 5% level. The giving of red rosella on dried dose 540 mg/bw and red rosella on dried dose 810 mg/bw can significantly prevent the increase of MDA serum in strain wistar rats exposed to cigarette smoke.


Keywords :
red rosella of dried, cigarette smoke, MDA


References


Daftar Pustaka

Alarco-Alanso, J., Zamilpa A, Aguilar, F.A., (2012) Pharmacological Charactererization of the Dieretic Effect of Hibiscus sabdariffa Linn (Malvaceae) extract. J. Ethnopharmacol 139: 751-6.

Badan Penelitian dan Pengembangan Kesehatan Kementerian Kesehatan RI., (2013) Riset Kesehatan Dasar (RISKESDAS) 2013. Jakarta www.litbang.depkes.go.id/sites/download/rkd2013/Laporan_Riskesdas2013.PDF (sitasi 20 November 2016).

Esa, N.M., Hern, F.S., Ismail, A., and Yee, C.I., (2010) Antioxidant Activity in Different Parts of Roselle (Hibiscus sabdariffa L.) Extracts and Potential Exploitation of the Seeds. Food Chemistry 122 (2010) 1055-1060.

Guyton dan Hall., (1997) Buku Ajar Fisiologi Kedokteran Edisi 9. Jakarta : Penerbit Buku Kedokteran EGC.

Halliwell, B., Gutteridge, J.M.C., (2007) Free Radicals in Biology and Medicine. Fourth edition. New York. Oxford University Press.

Kurnia, H., Permatasari, N., dan Subandi (2011) Pengaruh Ekstrak Jintan Hitam terhadap MDA dan Sel Spermatogonium Tikus Yang Dipapar Asap Rokok Kretek Subakut. Jurnal Kedokteran Brawijaya. Vol. 26, No.3.

Lopes, AG., Thiago SF., Renata TN., Manuella L., Karla MPP., Ari MS., Ricardo MB., Antonio JRS., Samuel SV., and Luis CP., (2013) Antioxidant Action Of Propolis On Mouse Lungs Exposed To Short-term Cigarette Smoke, BioorganicAnd Medicinal Chemistry 21 (2013) 7570 - 7577.

Muliartha, IKG., Endang S., dan Yuliawati (2009) Oral Consumption Of Combined Vitamin C dan E Repair Liver Damage Due To Subchronic Exposure To Cigarette Kretek. Jurnal Kedokteran Brawijaya. Vol XXIV, No.1.

Murray, R. K., Granner, D. K., & Rodwell, V. W., (2009) Biokimia Harper. Edisi 27. Jakarta: Buku Kedokteran EGC.

Park, EM., dkk., (1998) Oxidative Damaged Tissue of Rats Exposed to Cigarette Smoke. Free Radicals Biology Medicine.

Santi, S, W., (2006) Pengaruh Pemberian Dekok dan Instan Jahe (Zingiber officinate Rocs.) Terhadap Kadar MDA Serum, Hepar, dan Paru Tikus (Rattus norvegicus) Yang Dipapar Asap Rokok Kretek Sub Akut, skripsi. Fakultas Kedokteran Universitas Brawijaya. Malang. Hal 14-15.

Subono, FA (2007) Pengaruh Ekstrak Bayam (Amaranthus Tricolor L) Terhadap kadar MDA dan Aktivitas GSH Px Rattus Norvegicus Strain Wistar Yang Terpapar Asap Rokok. Program Studi Ilmu Kedokteran Dasar. Universitas Airlangga Surabaya.

Sofia dan Dinna., (2006) Antioksidan dan Radikal Bebas (online) www.chemistry.org

Tirtosastro, S dan Murdiyati, A.S., (2010) Kandungan Kimia Tembakau dan Rokok. Buletin Tanaman Tembakau, Serat dan Minyak Industri 2(1), April 2010:33-43.

Valvanidis A, dkk., (2001) A Comparative Study by Electron Paramagnetic Resonance of Free Radicals Species in The Main Steam and Side Stream Smoke of Cigarettes with Conventional Accate Filters and Biofilters, Medline Journal : England.

Voges, E., (2000). Tobacco Encyclopedia. Tabac Journal International, Mainz, Germany, 279p.

World Health Organization, (2008) WHO Report On The Global Tobacco Epidemic 2008. WHO publisher, New York: 3-8.


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